# TwoStep Therapeutics

Startup profile on Capital Atlas: https://capitalatlas.ai/startups/twostep-therapeutics

- Industry: Biotechnology
- Headquarters: San Carlos, US
- Website: www.twosteptx.com/
- Total disclosed funding: $69M
- Latest round: Series A (Sep 9, 2026)
- Investors on record: 10

## About

Founded in 2023 by Caitlyn Miller (CEO), Jennifer Cochran, Ronald Levy, and Carolyn Bertozzi, and based in California, US, TwoStep Therapeutics, Inc. is a biotechnology company that develops and offers targeted therapies across various solid tumors. In September 2026, TwoStep Therapeutics, Inc. raised USD 62.5 million in series A funding co-led by Insight Partners, Medical Excellence Capital, M Ventures, and Pfizer Venture Investments, with participation from  Stanford University, 2048 Ventures and NFX. The company aims to improve cancer treatment by extending precision medicines to a wider range of tumor types and patient populations with limited options. Their approach involves the use of clinically validated cytotoxic payloads and immunotherapy leveraging the modular PIP that offers multi-specific targeting to offer cancer therapies. The company intends to use the September 2026 funding to advance TS-104 through Phase 1 clinical trials, develop its radioligand program, and expand its next-generation peptide conjugate pipeline.

## Funding rounds

- Series A, Sep 9, 2026, $63M — Insight Partners, M Ventures, Pfizer Venture Investments, Stanford University, NFX, 2048 Ventures, Medical Excellence Capital
- Seed, Mar 1, 2024, $6.5M — Alexandria Venture Investments, GC&H Investments, NFX, 2048 Ventures, Arcadia Investment Partners

## Investors

- Alexandria Venture Investments (https://capitalatlas.ai/funds/alexandria-venture-investments) — Seed
- GC&H Investments (https://capitalatlas.ai/funds/gc-h-investments) — Seed
- NFX (https://capitalatlas.ai/funds/nfx) — Seed, Series A
- 2048 Ventures (https://capitalatlas.ai/funds/2048-ventures) — Seed, Series A
- Arcadia Investment Partners — Seed
- Insight Partners (https://capitalatlas.ai/funds/insight-partners) — Series A
- M Ventures (https://capitalatlas.ai/funds/m-ventures) — Series A
- Pfizer Venture Investments (https://capitalatlas.ai/funds/pfizer-venture-investments) — Series A
- Stanford University — Series A
- Medical Excellence Capital (https://capitalatlas.ai/funds/medical-excellence-capital) — Series A